Reversal of Myoblast Aging by Tocotrienol Rich Fraction Posttreatment

Abstract : Skeletal muscle satellite cells are heavily involved in the regeneration of skeletal muscle in response to the aging-related deterioration of the skeletal muscle mass, strength, and regenerative capacity, termed as sarcopenia. This study focused on the effect of tocotrienol rich fraction (TRF) on regenerative capacity of myoblasts in stress-induced premature senescence (SIPS). The myoblasts was grouped as young control, SIPS-induced, TRF control, TRF pretreatment, and TRF posttreatment. Optimum dose of TRF, morphological observation, activity of senescence-associated β-galactosidase (SA-β-galactosidase), and cell proliferation were determined. 50 μg/mL TRF treatment exhibited the highest cell proliferation capacity. SIPS-induced myoblasts exhibit large flattened cells and prominent intermediate filaments (senescent-like morphology). The activity of SA-β-galactosidase was significantly increased, but the proliferation capacity was significantly reduced as compared to young control. The activity of SA-β-galactosidase was significantly reduced and cell proliferation was significantly increased in the posttreatment group whereas there was no significant difference in SA-β-galactosidase activity and proliferation capacity of pretreatment group as compared to SIPS-induced myoblasts. Based on the data, we hypothesized that TRF may reverse the myoblasts aging through replenishing the regenerative capacity of the cells. However, further investigation on the mechanism of TRF in reversing the myoblast aging is needed.
Document type :
Journal articles
Complete list of metadatas

Cited literature [57 references]  Display  Hide  Download

https://hal.sorbonne-universite.fr/hal-01596040
Contributor : Gestionnaire Hal-Upmc <>
Submitted on : Wednesday, September 27, 2017 - 10:39:03 AM
Last modification on : Thursday, March 21, 2019 - 12:25:53 PM
Long-term archiving on : Thursday, December 28, 2017 - 12:57:53 PM

File

978101.pdf
Publisher files allowed on an open archive

Identifiers

Citation

Jing Lim, Wan Zurinah Wan Ngah, Vincent Mouly, Norwahidah Karim. Reversal of Myoblast Aging by Tocotrienol Rich Fraction Posttreatment. Oxidative Medicine and Cellular Longevity, 2013, 2013, pp.978101. ⟨10.1155/2013/978101⟩. ⟨hal-01596040⟩

Share

Metrics

Record views

360

Files downloads

84