Pathogenic potential of Escherichia coli clinical strains from orthopedic implant infections towards human osteoblastic cells

Abstract : One sentence summary: This study describes the interaction of Escherichia coli clinical strains with an osteoblastic cell line, showing no internalization unlike Staphylococcus aureus, but a strong cytotoxicity of the Hly-A producing strains. Editor: Patrik M. Bavoil ABSTRACT Escherichia coli is one of the first causes of Gram-negative orthopedic implant infections (OII), but little is known about the pathogenicity of this species in such infections that are increasing due to the ageing of the population. We report how this pathogen interacts with human osteoblastic MG-63 cells in vitro, by comparing 20 OII E. coli strains to two Staphylococcus aureus and two Pseudomonas aeruginosa strains. LDH release assay revealed that 6/20 (30%) OII E. coli induced MG-63 cell lysis whereas none of the four control strains was cytotoxic after 4 h of coculture. This high cytotoxicity was associated with hemolytic properties and linked to hlyA gene expression. We further showed by gentamicin protection assay and confocal microscopy that the non-cytotoxic E. coli were not able to invade MG-63 cells unlike S. aureus strains (internalization rate <0.01% for the non-cytotoxic E. coli versus 8.88 ± 2.31% and 4.60 ± 0.42% for both S. aureus). The non-cytotoxic E. coli also demonstrated low adherence rates (<7%), the most adherent E. coli eliciting higher IL-6 and TNF-α mRNA expression in the osteoblastic cells. Either highly cytotoxic or slightly invasive OII E. coli do not show the same infection strategies as S. aureus towards osteoblasts.
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Foodborne Pathogens and Disease, Mary Ann Liebert, 2015, 73 (8), ftv065 (Epub 2015 Sep 1). 〈10.1093/femspd/ftv065〉
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Lise Crémet, Alexis Broquet, Bénédicte Brulin, Cedric Jacqueline, Sandie Dauvergne, et al.. Pathogenic potential of Escherichia coli clinical strains from orthopedic implant infections towards human osteoblastic cells. Foodborne Pathogens and Disease, Mary Ann Liebert, 2015, 73 (8), ftv065 (Epub 2015 Sep 1). 〈10.1093/femspd/ftv065〉. 〈inserm-01792672〉

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